Trypelectase recombinant trypsin is specifically designed for the production of vaccines and viral vectors, providing highly efficient, animal-origin free products meeting high quality standards. The amino acid sequence of this trypsin is identical to that of the porcine pancreatic trypsin, and is produced by recombinant expression in Escherichia coli E. coli. Recombinant trypsin produced in GMP Grade environment can efficiently digest adherent cells without causing harm, and its quality meets the pharmacopoeia requirements.
Properties:
Item Description Sequence Source Pigs Appearance White crystalline powder Molecular weight 23.4kDa Purity β-trypsin≥70%，α-trypsin ≤20% Specific activity ＞3800 (U/mg protein) Endotoxin ≤5 EU/mg Residual Exogenous DNA ≤ 100 pg/mg Microbial Limit ＜5 cfu/g Optimal PH 6.8-7.3 Cofactor CaCl2 Grade & Storage Conditions -20℃
Definition of active unit: when hydrolyzing BAEE at 25°C, pH 7.6, reaction system of 3.2 mL (optical path = 1 cm), one active unit will produce an increase of 0.003 in A253 (absorbance value at 253 nm) per minute.
Features:
Manufactured in a GMP-compliant facility in compliance with pharmacopoeial standards
Recombinant production, animal-origin free raw materials
High purity and high specific activity
Application case study:
Cell dissociation test:
Cell Vitality Concentration Dissociation time CT26.WT 98% 0.05%（m/V） 3min±0.04 HEK293FT 97% 0.05%（m/V） 3min±0.04
Images of cell dissociation process by Trypelectase recombinant porcine trypsin and cell adherence after 24h of passaging:
CT26.WT and HEK293FT cells were well dissociated after 3 min of enzymolysis by Trypelectase recombinant porcine trypsin, and the cell viability results indicated minor damage to cells which will not affect cell passaging.
Transportation and Storage:
When stored at -15°C ~ -25°C, the validity period is 2 years.
Recommended Condition of Use: At room temperature, or preheated
Usage:
Procedure of adherent cell dissociation:
Reagents and consumables: Trypsin dry powder, solving liquid, PBS (pH 7.4), 0.22 μm filter membrane
1.Take the Trypelectase trypsin dry powder reagent vial from the refrigerator and restore to room temperature (about 30 min).
2.Solving liquid: 1 mM HCl and 5% sorbitol, pH 3.0, filter through a 0.22 μm membrane for sterilization.
3.Weigh the dry powder and gently reconstitute in the solving liquid to a trypsin concentration of 10 mg/mL in the solution.
4.Preparation of the Trypelectase trypsin buffer for cell dissociation:
Mix the mother liquor with sterile PBS (pH7.4) at 1:20 (v/v) to a pancreatin cell dissociation solution of 0.5mg/mL at pH7.4. If incubation at 37°C is required, PBS can be incubated at 37°C in advance before mixing with the mother liquor.
Order Imformations：
Cat. No. | Name | Size |
CYG005F0001 | Trypelectase Recombinant Trypsin | 1g |
CYG005F0010 | Trypelectase Recombinant Trypsin | 10g |
