Trypelectase, a recombinant bovine trypsin, has amino acid sequences identical to that of bovine pancreatic trypsin. It is manufactured by recombinant expression in Escherichia coli (E.coli). Thanks to its high activity and purity, Trypelectase is widely used in the preparation of third-generation insulin in China and overseas. overseas.
Properties:
Item | Description |
Sequence Source | Bovine |
Appearance | White crystalline powder |
Molecular weight | 23kDa |
Specific activity | 200 (U/mg protein TAME) |
Endotoxin | ≤5 EU/mg |
Residual Exogenous DNA | ≤ 100 pg/mg |
Microbial Limit | ＜5 cfu/g |
Grade & Storage Conditions | -20℃ |
Definition of active unit: when hydrolyzing TAME at 30°C, pH 8.1, reaction system of 3.0 mL (optical path = 1 cm), one active unit (U) will produce an increase of 0.001 in A253 (absorbance value at 253 nm) per minute.
Features:
Manufactured in a GMP-compliant facility in compliance with pharmacopoeial standards
A halal biologics with high specificity in insulin cleavage
Manufactured by recombinant expression, free of animal-derived materials, free of exogenous viral contamination
Characterization by SDS-PAGE:
Protein molecular weight of about 15 kDa by non-reduced and reduced SDS-PAGE; no protein impurity band (5μg Sample)
Enzyme activity assay:
Enzyme activity: 212 U/mg protein; Definition of active unit: when hydrolyzing TAME at 30°C, pH 8.1, reaction system of 3.0 mL (optical path = 1 cm), one active unit (U) will produce an increase of 0.001 in A253 (absorbance value at 253 nm) per minute.
Transportation and Storage:
When stored at -15°C ~ -25°C, the validity period is 2 years.
Recommended Condition of Use: At room temperature, or preheated
Usage: Dissolve in ddH2O before use.
Order Imformations：
Cat. No. | Name | Size |
CYG004F0001 | Trypelectase Recombinant Bovine Trypsin | 1g |
CYG004F0010 | Trypelectase Recombinant Bovine Trypsin | 10g |
