D-luciferin potassium salt

The luminescence produced by the reaction of luciferase with luciferin substrate is mostly used in bioluminescence imaging. The use of luciferase gene to label cells and the detection of fluorescence intensity after injection of luciferase substrate allow real-time monitoring of the growth of target cells and the drug medication effect. Since ATP is also involved in the fluorescence reaction, the effect of ATP on the system can also be introduced to indicate energy or life state.

Principle: Luciferase catalyzes the oxidative decarboxylation of D-luciferin potassium salt in ATP, Mg²⁺ and O₂ , and emits blue-green fluorescence with a wavelength of 560 nm on average. The number of photons produced by adding excess luciferin potassium salt is positively correlated with the concentration of luciferase. Easy operation and accurate quantification make it very widely used in biotechnology, especially in the field of in vivo imaging and reporter gene technology.

Properties:

High purity (HPLC-verified purity ≥99%)
Excellent bioluminescence performance
High solubility (> 40 mg/mL)

Free acidluciferin has poor solubility,while those in potassium salt form showed good water solubility. The high purity D-luciferin potassium salt developed by Chaselection has a solubility in water up to 40 mg/mL or above.

Product Application:

In vivo imaging
Reporter gene assay
Cell viability testing

Procedures for use in vivo imaging:

Example of vivo imaging:

Order Imformations：

Cat.NO.	Name	Size
CY057F0025	D-luciferin potassium salt	25mg
CY057F0100	D-luciferin potassium salt	0.1g
CY057F1000	D-luciferin potassium salt	1 g

D-luciferin potassium salt Brochure.pdf

D-luciferin potassium salt Manuel.pdf

Q:What is the purity of D-fluorescein potassium salt? Does purity have an impact on the experiment?

A:The purity of D-fluorescein potassium salt is ≥ 99%. The higher the purity of fluorescein, the lower the corresponding pollutant content. On the contrary, the higher the concentration. Excessive concentration of pollutants will inhibit certain enzymes and biological processes in cells, which may lead to deviation in experimental results and may cause harm to experimental animals.

Q:What is the solubility of D-fluorescein potassium salt? Is its solution stable?

A:The solubility of D-fluorescein potassium salt is above 40 mg/ml, and its aqueous solution is stored at -80 ℃ for a short period of time, which has no significant impact on the experimental results. For experiments that are sensitive to certain conditions, in order to control the variable factors in the experiment, it is recommended to prepare the reaction solution fresh. For example, low luciferase levels and non optimal reaction conditions require high-quality substrates for the reaction.

Q:What is the recommended dosage of D-fluorescein potassium salt?
A:Live imaging: at 10 μL/g Determine the injection volume based on body weight. Each mouse receives 150 mg of D-fluorescein potassium salt per kg body weight (e.g. for mice weighing 10 g, 100 μL injections are required to provide 1.5 mg of D-fluorescein potassium salt).
In vitro imaging: generally used 150 μg/ml working solution for in vitro cell imaging.

Tips: The injection method, animal type, and weight can all affect signal emission. It is necessary to explore appropriately and wait for the fluorescence signal to reach the strongest stable plateau period before using corresponding instruments for imaging analysis.

Q:How to store the prepared D-fluorescein potassium salt solution?
A:The working solution is prepared and used immediately. Ideally, the prepared storage solution can be stored for a short period of time at -20 ℃. If necessary, the fluorescent solution can be stored at -20 ℃ for up to 3 we