Trace amounts of nuclease residues may be found in treated biologics, and these trace residues may have an impact on the subsequent application of the biologics, thus the amount of nuclease residues in the biologics is one of the important indicators of the quality of the biologics. Currently, nuclease residue assays are mostly based on the ELISA method. The Chaselection ELISA Kit of Pannarase Nuclease is designed for the residue assay of Pannarase nuclease, and is also compatible with a variety of Benzonase nucleases on the market.
Properties:
Linear Range
The wide linear range from 0.2 ng/mL to 10 ng/mL is suitable for routine working concentrations of 10-20 U/mL, allowing for the detection of residues without repeated tedious dilutions.
ELISA kit accuracy test
High recovery results were obtained after the addition of Pannarase nuclease at three concentrations (low, medium and high levels).
ELISA kit precision test
The CV% results of the control SR1 (Pannarase nuclease standard) and 1% BSA (Pannarase nuclease standard spiked) were all below 15% at three concentrations of high, medium and low levels, indicating high precision and repeatability.
Detection of nucleases of different brands
The ELISA Kit of Pannarase Nuclease is applicable to a variety nucleases of different brands, achieving high stability and repeatability of the detection results.
Features:
Direct antibody coating, good inter-run consistency
High sensitivity and wide linear range
Transportation and Storage:
Transport on dry ice. When stored at 2℃ ~ 8℃, the validity period is 1 year.
Order Imformations：
Cat. No. | Name | Size |
| CYG003RKIT | ELISA Kit of Pannarase Nuclease | 1KIT 96Tests |
Q: High background or high negative control value
A:(1) Insufficient washing of the plate: Inject detergent into the reaction hole for thorough washing, and thoroughly pat dry the liquid in the hole
(2) Excessive enzyme binding: Check the enzyme dilution and dilute according to the instructions
(3) Substrate contamination: Before adding the substrate, check if it is transparent and colorless. Do not use a substrate that turns blue, and retest with a new substrate
(4) Negative control hole is contaminated by positive control: Be careful not to spill the washing solution out of the hole during washing, and do not mix the liquid in the negative and positive control holes together
(5) Mixing different batches of reagents: Check the batch number of reagents, do not use different batches of reagents
Q: Weak color signal
A:(1) Expiration of reagents: Check the validity of the reagent kit and do not use expired reagents
(2) Short incubation time: Incubate according to the time specified in the instruction manual
(3) Reagent contamination: Check if the reagent is contaminated and do not use contaminated reagents
(4) Mismatch of enzyme-linked immunosorbent assay filter: Check the enzyme-linked assay settings to see if the filter matches
(5) Insufficient balance of reagent kit: Ensure that the reagent kit is balanced to room temperature before testing
(6) Insufficient color development time: increase substrate color development time
Q: No color signal
A:(1) Detection of antibody, enzyme, or chromogenic agent omission: Check the operation process of the experiment and repeat the experiment
(2) Enzymes contaminated with sodium azide: Please use a newly prepared reagent
(3) Incorrect reagent addition sequence: recheck the addition sequence, process, and repeat the test
Q:The standard curve is good, but there is no signal from the sample hole
A:(1) Low target content in the sample or no target in the sample: Set up a positive control and repeat the experiment
(2) Sample matrix effect impact detection: retest after re diluting the sample
Q:Good standard curve but high sample signal
A:The content of the substance to be tested in the sample exceeds the range of the standard curve: dilute the sample again and retest it
Q:edge effect
A:Uneven incubation temperature: Use new sealing tape at each step of incubation to avoid incubating in areas with significant temperature changes, and do not stack reaction plates
