Potassium fluorescein salts are widely used in the study of in vivo bioluminescence imaging. Recently, we received a question from a researcher that when using D-fluorescein potassium salt to prepare reserve solution, sometimes turbidity may occur. How can this be resolved?The general solution for this situation is to add a small amount of potassium carbonate solution for dissolution, but it may prolong the signal reception time.
Is there a better solution?
Yes! Use D-fluorescein potassium salt with higher effective purity.
If the purity of D-fluorescein potassium salt can reach over 99%, the solubility in room temperature aqueous solution can reach 41mg/mL However, many products have a solubility of only 20mg/mL and may even experience turbidity, which indirectly reflects that the effective purity is not enough and will greatly affect the experimental process.
Chaselection D-fluorescein potassium salt has high purity (HPLC>=99%) and a solubility of over 40mg/ml. It has good water solubility and lipid solubility, and can be better applied in research such as in vitro living cells and in vivo bioluminescence imaging compared to D-fluorescein (free acid).
